Figure 1

Cytotoxicity towards vSAG7⁺ tumour cells generated by stimulated Vβ6⁺ T cells in vitro. (A) Vβ6⁺ T cells were isolated from B10.D2 mice which were either immunised with ESb-MP cells or remained untreated (1° in vivo). Then, Vβ6⁺ T cells were stimulated for 4 days either with irradiated vSAG7⁻ Eb or with vSAG7⁺ ESb-MP cells and APC (2° in vitro) and tested for cytotoxicity towards Eb or ESb-MP target cells (effector : target ratio 10 : 1) in a 4 h ⁵¹Cr-release assay. The mean±standard deviation of triplicates of two independent experiments are shown. (B) CD4⁺Vβ6⁺, CD8⁺Vβ6⁺ and total Vβ6⁺ T cells were isolated from non-immunised B10.D2 mice, purified and stimulated with irradiated ESb-MP and APCs for 4 days. Then effector cells were co-cultured with target ESb-MP cells for 2 h, and tumour cell lysis was determined by PI staining and FACScan analysis. The mean±standard deviation from 2–4 independent experiments are shown.