Figure 2

Stimulated Vβ6⁺ T cells induce apoptosis in vSAG7⁺ ESb-MP tumour cells. In vitro stimulated Vβ6⁺ T cells were co-incubated with ESb-MP cells in a ratio of 10 : 1 for different periods of time and stained either with Annexin V-FITC and 1 μg ml⁻¹ PI (A) or were treated with a hypotonic solution containing 50 μg ml⁻¹ PI overnight to determine DNA-fragmentation (B). Untreated cells showed no changes in the amount of apoptotic cells during coincubation. Apoptosis was determined by FACScan analysis. One representative experiment out of four (A) or three (B) independent experiments is shown.