Figure 4
Vβ6+ T cell-induced apoptosis in ESb-MP cells does not involve changes in Δψm. ESb-MP cells remained untreated (light line) or were cocultured with stimulated Vβ6+ T cells in a effector : target ratio of 10 : 1 for 4 h (dark line). As a control, Jurkat cells were left untreated (light line) or treated with anti-APO-1 mAb/Protein A for 12 h (dark line). Afterwards, ESb-MP cells and Jurkat cells were either analysed for DNA-fragmentation (FL3) or for changes in Δψm by JC1 staining (FL1). Upon incubation of Jurkat cells with anti-APO-1 mAb, FL2 did not significantly change, whereas FL1, which indicates the formation of dye monomer, increased due to the reduction of Δψm. This increase is taken as a measure for the loss of Δψm. One representative experiment out of three is shown.
