Figure 2
Expression of SIR-T8 in normal and tumoral thyroid cells. Total RNA (5 μg per lane) was size-fractionated on denaturing formaldehyde agarose gel, blotted onto nylon filters (hybond-N, Amersham) and probed with SIR-T8 cDNA. RNA was fractioned from the following sources: lane 1: normal thyroid primary culture cells; lane 2: NIM cell line; lane 3: TPC-1 cell line; lane 4: NPA cell line; lane 5: WRO cell line; lane 6: FRO cell line; lane 7: ARO cell line. A GAPDH probe was used as internal control for uniform RNA loading. Lower panel: densitometric analysis of SIR-T8 expression. Bars present the mean±s.d. of the results obtained in three experiments. *P<0.0001 vs HTC2, #P<0.005 vs NPA and **P<0.001 vs HTC2.
