Figure 4
From: Cell cycle phase perturbations and apoptosis in tumour cells induced by aplidine
Effects of 1 h Aplidine exposure on the cell cycle phase distribution of Molt-4 cells evaluated at 0, 4, 8, 12, 24, 29, 36 and 48 h after drug-washout. During the last 15 min 10 nM Aplidine treatment, 20 μM BrdUrd was added to the cells, then the cells were washed with PBS and drug-free medium was provided. (A) Biparametric BrdUrd/DNA analysis of (a) control cells; and (b) Aplidine treated cells. (B) DNA histograms of (a) control cells (whole population); (b) Aplidine treated cells (whole population); (c) BrdUrd-negative control cells; (d) BrdUrd-negative Aplidine treated cells; (e) BrdUrd-positive control cells; and (f) BrdUrd-positive Aplidine treated cells. The data are representative of three independent replicates.
