Figure 6
From: Cell cycle phase perturbations and apoptosis in tumour cells induced by aplidine
(A) Immunoblot analysis of Cdk2, Cdc2, Cdk4, cyclin B1, D1 and E, p21, p16, p53, and pRb expression in Molt-4 cells treated for 1 h with 10 nM Aplidine (three independent replicates). Molt-4 extracts were collected before and after treatment and, at 4, 8, 24 h after drug washout. One hundred μg of protein extracted from cells were immunoblotted with specific antibodies as described in Materials and methods. (B) (a) Histone H1 phosphorylation in Molt-4 cells lysates immunoprecipitated with Cdk2 antibodies. Cells were exposed for 1 h to 10 nM Aplidine (0 h) and then incubated in drug free medium (4 and 24 h after drug-washout) (three independent replicates). (b) Western blot analysis of Cdk2 and cyclin E in Cdk2 immunoprecipitates (three independent replicates).
