Figure 5
From: Investigations on a clinically and functionally unusual and novel germline p53 mutation

Induction of apoptosis. The pC53-SN3 plasmid containing the p53 insert of interest was transfected into Saos-2 cells, which were washed after 16 h and reincubated for 72 h. The results show that the 7 base pair mutation retained about 65% of the apoptotic ability of WT p53. 344P, the non-functional mutant only retained about 20% apoptotic ability. Statistical analysis showed that the 7 base pair mutation was significantly different from both 344P and WT, with P<0.02.