Figure 1
Expression of antisense RNA. Northern blots are shown of the cell lines SKM13-Tet-On (A and B) and of Bro-Tet-On (C and D). Total RNA was extracted 2 days after transient transfection of six different antisense and sense constructs for translation initiation factor eIF-4A1 subcloned behind a tetracycline-inducible promoter (names of constructs are given in the top). Cell cultures grown with doxycycline (+) were compared with cultures grown without doxycycline (−). Northern blots were hybridised with cDNA probes specific for eIF-4A1 (A and C) and for β-actin (B and D), respectively. The position and length of the endogeneous mRNAs for eIF-4A1 (1850 N and 1550 N), of β-actin (1800 N), and of the different plasmid-derived RNAs is indicated at the left margin.
