Figure 3
Reduced proliferation after induction of AS-2x-central in stably transfected cell clones. The cell line Bro-Tet-On was stably transfected with the antisense construct AS-2x-central. Five individual cell clones (499) as well as the parent cell line Bro-Tet-On were analysed 2 days after induction with doxycycline. (A) Amount of endogeneous mRNA for eIF-4A1, as determined by Northern blotting, densitometry and normalisation with β-actin mRNA (median values of two independent experiments). (B) Relative proliferation rates were calculated as the ratio between cultures induced with doxycycline versus non-induced cultures (in %), and the bars represent deviations from 100%. Each of the bars corresponds to mean values of at least three independent experiments of each 60 counted wells (180 wells). Significant reduction of proliferation is indicated by asterisks. According to Student's t-test, P-values were <0.03 (499-22), <0.02 (499-41), <0.002 (499-62).
