Figure 4
From: Apoptotic mechanisms in T47D and MCF-7 human breast cancer cells
Staurosporine induced PARP cleavage in MCF-7 and T47D cells. Both (A) MCF-7 and (B) T47D cells were either treated with 1 μM STS for the times indicated or pre-incubated with 100 μM z-VAD-fmk for 1 h prior to STS treatments. Cell lysates (40 μg protein) were prepared and samples subjected to 8% SDS–PAGE followed by Western transfer and probed with PARP antibody (full length protein is 113 kDa, and cleaved fragment is 85 kDa). The results are representative of three independent experiments.
