Figure 6
From: Apoptotic mechanisms in T47D and MCF-7 human breast cancer cells
Staurosporine induced cytochrome c release into the cytosol of (A) MCF-7 and (B) T47D cells. After treatment with 1 μM STS for the times indicated cytosolic extracts were prepared and 30 μg protein subjected to 14% SDS–PAGE followed by Western blot analysis with anti-cytochrome c antibody (Cyt c) and cytochrome c oxidase subunit II (Cyt Ox). Whole cell lysate (WCL) was used as a positive control for the cytochrome c oxidase. Results are representative of four independent experiments.
