Figure 3
Photosensitisation of DT-exposed cells. LNCaP cells pretreated for 72 h with 10−7 M R1881 (▵) and control cells (O) were incubated for 4 h with 0.3 mm ALA and irradiated. PpIX-mediated phototoxicity was significantly enhanced in androgen-pretreated cells (▵) using (A) MTT conversion and (B) colony formation as endpoints. Differentiated (▵) and control cells (O) containing similar amounts of PpIX (C) showed a small decrease in photosensitivity in the differentiated cells. (D) Pretreatment with 10−7 M Ro-26-2198 for 96 h before photosensitisation resulted in increased cytotoxicity in Ro-26-2198-treated cells (▵) as compared to control cells (O), as assessed by MTT conversion. All data points are mean±s.d. values of duplicate samples.
