Figure 3
From: Regulation of a rat VL30 element in human breast cancer cells in hypoxia and anoxia: role of HIF-1
(a) Mean (±s.e.m.) fold induction (top panel), firefly LUC (middle panel) and Renilla LUC (bottom panel) detected in MCF-7 cells following transfection with either the pGL3 Promoter vector or this containing trimerised versions of the wt SARE or mutant forms of this (M1 and M2; see Table 1a, b). Cells were exposed to 0, 0.5, 1 or 21% O2 for 16 h. *P<0.05 with respect to the same trimer at 0.5% O2 (unpaired t-test). Pooled data from four experiments are given. (b) Mean (±s.e.m.) fold induction (top panel), firefly LUC (middle panel) and Renilla LUC (bottom panel) in MCF-7 cells following transfection with either the pGL3 Promoter vector alone or this containing trimers of M1 or M3 following exposure to 0, 0.5, 1, or 21% O2 for 16 h. *P<0.05 with respect to the same trimer at 0.5% O2 (unpaired t-test). Pooled data from four experiments are given.
