Figure 8
From: Regulation of a rat VL30 element in human breast cancer cells in hypoxia and anoxia: role of HIF-1
Mean (±s.e.m.) standardised LUC light units (ratio of firefly LUC/Renilla LUC readings) in either wild type (HIF-1 +/+) or HIF-1α knockout (HIF-1 −/−) CHO cells (Wood et al, 1998) following transfection with a pGL3 Promoter plasmid expressing firefly LUC under control of a trimer of the SARE, following exposure to 21% O2, 0.5% O2, or 0% O2 for 16 h. The SARE inducibility is lost in the HIF-1α knockout (HIF-1 −/−) CHO cells. P=0.003 with respect to HIF-1 +/+ cells at 21% O2, or the same oxygen tensions for HIF−/− cells.
