Figure 2

Immunhistochemical staining of serial tissue sections of a nodular melanoma, tumour thickness according to Breslow 1.4 mm, Clark's level IV. The PTEN/MMAC1 protein was detected in melanoma cells and was not restricted to tumour associated macrophages. (a) Hematoxylin Eosin, (b) HMB45, (c) CD68, (d) PTEN/MMAC1 staining (all magnification 25 ×, nickel enhanced DAB reaction resulting in black signals).