Figure 2

Intracellular fluorescence spectra of LY alone (A). Intracellular fluorescence spectra (B) and fluorescence topographic profiles (C) of MCF-7 cells incubated overnight with 125 μg ml−1 LY and Foscan® (1 μg ml−1, 3 h). The spectrum in (B) was recorded at position 16.6 μm on the X-axis of the profile in (C). Fluorescence topographic profiles in (C) were recorded at 550 nm (LY) and at 654 nm (Foscan®). Excitation wavelength 435 nm, objective magnification × 40, exposure time 4 s.