Figure 5
Intracellular fluorescence spectra of DiOC6 alone (A). Intracellular fluorescence spectra (B) and fluorescence topographic profiles (C) of MCF-7 cells incubated for 3 h with 1 μg ml−1 Foscan® and then with 2 μg ml−1 DiOC6 for 15 min. The spectrum in (B) was recorded at position 5.70 μm on the X-axis of the profile in (C). Fluorescence topographic profiles in (C) were recorded at 545 nm (DiOC6) and at 654 nm (Foscan®). Excitation wavelength 435 nm, objective magnification × 100, exposure time 4 s.
