Figure 3
From: Temozolomide induces apoptosis and senescence in glioma cells cultured as multicellular spheroids
TUNEL labelling. Cross-sections of paraffin-embedded spheroids, stained for fragmented DNA by TUNEL. The more loosely associated U87 spheroids contain significantly more apoptotic nuclei 2 days after temozolomide treatment (A, B). It takes about 6 days until there is a significant number of apoptotic nuclei labelled in GaMG spheroids (C, D). Nuclei are counterstained with nuclear fast red.
