Figure 4
From: Temozolomide induces apoptosis and senescence in glioma cells cultured as multicellular spheroids
DNA fragmentation. Laddering phenomenon of fragmented DNA resolved on a 1.5% agarose gel. Alkylation of DNA by temozolomide results in induction of apoptosis in U87 cells during the first 48 h postexposure. After 7 days, untreated U87 cells also undergo apoptosis because of unfavourable culture conditions. GaMG is not initiating apoptosis after 2 days and a slight induction is observed after 7 days in culture.
