Figure 3

(A) Western blot analysis of BC2 (i), BC4 (ii) and ST2146 (iii) antibodies. A: pTn28; B: A–D fragment; C: tenascin. (B) Competitive ELISA. Biotinylated ST2146 was mixed with increasing concentrations of ST2146, BC4 or a not related IgG1 as competitors and plated on tenascin-coated plates. Binding was measured after addition of horseradish peroxidase–streptavidin and related chromogenic substrate.