Table 1 A summary of patient details, including operation and preoperative serum CEA levels, with T lymphocyte population phenotypes after retroviral transduction and expansion

From: T lymphocytes isolated from patients with advanced colorectal cancer are suitable for gene immunotherapy approaches

Patient (age in years)

ScFv fused to CD3ΞΆTDGA

Preoperative CEA ( ΞΌ g lβˆ’1)

Operation

% CD3+

% CD3+, GFP+

% CD8+, GFP+

% CD4+, GFP+

Ratio of transduced CD8+ : CD4+

Ratio of total CD8+ : CD4+ T cells

1 (70)

Mock

35

Left hepatectomy

99

0

0

0

β€”

11.5 : 1

Β 

MFE23

Β Β 

99

20

12

3

4 : 1

8.9 : 1

Β 

D29

Β Β 

99

15

17

3

5.7 : 1

9.1 : 1

2 (66)

Mock

6

Right hepatectomy

99

0

0

0

β€”

4.2 : 1

Β 

MFE23

Β Β 

99

5

5

2

2.5 : 1

4.9 : 1

Β 

D29

Β Β 

99

7.5

7

1

7 : 1

4.5 : 1

3 (64)

Mock

1

Right hepatectomy

96

1

0

0

β€”

2.3 : 1

Β 

MFE23

Β Β 

91

21

19

16

1.2 : 1

1.5 : 1

Β 

D29

Β Β 

90

13

9

6

1.2 : 1

1.5 : 1

4 (70)

Mock

9

Right hepatectomy

99

0

0

0

β€”

1.9 : 1

Β 

MFE23

Β Β 

94

21

19

8

2.4 : 1

2.1 : 1

Β 

D29

Β Β 

97

9

11

5

2.2 : 1

2.2 : 1

5 (42)

Mock

155

Left hepatectomy+segmentectomy

88

1

2

1

2 : 1

1.3 : 1

Β 

MFE23

Β Β 

93

33

17

13

1.0 : 1

1.4 : 1

Β 

D29

Β Β 

89

23

20

14

1.4 : 1

1.4 : 1

6 (73)

Mock

>1500

Right Hepatectomy

89

0

3

0

β€”

10 : 1

Β 

MFE23

Β Β 

88

11

10

1.5

6.7 : 1

4.3 : 1

Β 

D29

Β Β 

87

4

5

1

5 : 1

7.6 : 1

7 (78)

Mock

6

Inoperable – extra-hepatic disease

99

0

0

0

β€”

1.9 : 1

Β 

MFE23

Β Β 

99

23.9

15.6

7.9

2 : 1

1.8 : 1

Β 

D29

Β Β 

99

2

1

0.3

3.3 : 1

1.8 : 1

8 (66)

Mock

4

Inoperable – extra-hepatic disease

99

0

0

0

β€”

8.8 : 1

Β 

MFE23

Β Β 

99

19.9

15.5

1.9

8.1 : 1

7.0 : 1

Β 

D29

Β Β 

99

2

1.3

0.2

6.5 : 1

8.4 : 1

9 (66)

Mock

24

Right hepatectomy

99

0

0

0

β€”

7.4 : 1

Β 

MFE23

Β Β 

99

25

17

3

5.7 : 1

8.5 : 1

Β 

D29

Β Β 

99

20

19

2

9.5 : 1

8.2 : 1

10 (57)

Mock

156

Left hepatectomy

99

2

0

0

β€”

4.2 : 1

Β 

MFE23

Β Β 

99

14

18

3

6 : 1

4.0 : 1

Β 

D29

Β Β 

99

9

10

1

10 : 1

4.6 : 1

  1. All 10 patients recruited were due to undergo surgery for colorectal hepatic metastases. All the metastases removed or biopsed were confirmed as adenocarcinoma of colorectal origin by histological examination. T lymphocytes were isolated from these patients 2–3 weeks prior to surgery and were transduced with recombinant retroviruses encoding either MFE23.CD3ΞΆTDGA or D29.CD3ΞΆTDGA chimeric receptors. After expansion using an IL-2-based protocol, the T lymphocyte populations were analysed for the expression of the EGFP marker gene and for the surface markers CD3, CD4 and CD8 by staining with PE-conjugated antibodies and subsequent flow cytometric analysis using a FACscan (Becton Dickenson) with winMDI software.
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