Figure 2
From: Reduced endocytosis and altered lysosome function in cisplatin-resistant cell lines
Reduced accumulation of Texas Red dextran-10, a fluid-phase marker, in CP-r cells. (A, B) Cells were incubated with 10 mg ml−1 of Texas Red dextran-10 at 37°C for 2 h, then monitored under a laser scanning confocal microscope (Bio-Rad, Molecular Probes, Eugene, OR, USA) at a x600 magnification. (C, D) A time course of Texas Red dextran-10 accumulation was performed by FACS analysis. Cells were incubated with 3 mg ml−1 of Texas Red dextran-10 at 37°C for up to 7 h. (E) A semiquantitative analysis based on the data of Figure 2C and D.
