Figure 4
From: Reduced endocytosis and altered lysosome function in cisplatin-resistant cell lines
Kinetics of human procathepsin L secretion and processing in KB-3-1 (A) and KB-CP20 (B) cells. Cells were radiolabelled briefly for 30 min with Trans-35S-label and chased for varying time periods (0 – 240 min, as shown on the top of the gel panels A and B) in serum-free medium. At the end of each time period, medium was saved and cells were lysed after washing with ice-cold PBS. The radiolabelled human procathepsin L was immunoprecipitated using a polyclonal antibody. The immunoprecipitates were resolved on SDS – PAGE and subjected to fluorography. The values given on the left side of the panels indicate the molecular weight of different forms of cathepsin L in kDa.
