Figure 2
Schematic representations of the structures of CPG2(Q)3-H6 and fusion proteins. SPerb and SPv: signal peptides of c-erb B2 and VEGF, respectively. CPG2 23-415: amino acids 23–415 of CPG2, showing the relative positions of the three asparagine to glutamine mutations (Q) required to avoid glycosylation and concomitant loss of CPG2 activity. H6: oligohistidine tag for purification by nickel-agarose chromatography. VEGF165, VEGF109, VEGF115, and VEGF161: amino acids 1–165, 109, 115, and 161, respectively, of VEGF165. Black box: the relative position of the heparin-binding sequences of the longer forms of VEGF.
