Table 1 Structures of the fusion proteins

From: A novel vascular endothelial growth factor-directed therapy that selectively activates cytotoxic prodrugs

Protein

Predicted primary sequence

M r (e)

M r (o)

CPG2(Q)3-H6

(c-erb B2 aa 1-27)-GS-(CPG2(Q)3 aa 23-415)-EF(H)6AS

43 731

45 000

CPG2(Q)3-VEGF165

(c-erb B2 aa 1-27)-GS-(CPG2(Q)3 aa 23-415)-EF(G)5TA-(VEGF aa 1-165)

62 272

59–61 000

CPG2(Q)3-VEGF161-H6

(c-erb B2 aa 1-27)-GS-(CPG2(Q)3 aa 23-415)-EF(G)5TA-(VEGF aa 1-161)-EF(H)6AS

63 215

60–62 000

CPG2(Q)3-VEGF109

(c-erb B2 aa 1-27)-GS-(CPG2(Q)3 aa 23-415)-EF(G)5TA-(VEGF aa 1-109)

55 657

53–55 000

CPG2(Q)3-VEGF109-H6

(c-erb B2 aa 1-27)-GS-(CPG2(Q)3 aa 23-415)-EF(G)5TA-(VEGF aa 1-109)-EF(H)6AS

57 135

54–59 000

VEGF161-CPG2(Q)3-H6

(VEGF aa -26-161)-GS-(CPG2(Q)3 aa 23-415)-EF(H)6AS

61 710

61–63 000

VEGF115-CPG2(Q)3-H6

(VEGF aa -26-115)-GS-(CPG2(Q)3 aa 23-415)-EF(H)6AS

56 386

55–61 000

  1. Sequences of linkers between VEGF and CPG2-derived moieties and of oligohistidine tags for purification by nickel-agarose chromatography are given in the one-letter amino-acid code (22); Mr (e), expected relative subunit molecular weight; Mr (o), observed subunit molecular weight; aa, amino acids. c-erb B2 aa 1–27 encode the c-erb B2 signal peptide. VEGF aa -26-1 encode the VEGF signal sequence.
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