Table 3 Binding characteristics of fusion proteins between CPG2(Q)3 and VEGF expressed in insect cells

From: A novel vascular endothelial growth factor-directed therapy that selectively activates cytotoxic prodrugs

Protein

ELISA Kd (n M)

Competition RIA Kd (n M)

50% binding t 50 (s)

CPG2(Q)3-VEGF109-H6

0.75 (±0.05)

1.3 (±0.1)

332

VEGF161-CPG2(Q)3-H6

1.06 (±0.66)

3.3 (±0.3)

330

VEGF115-CPG2(Q)3-H6

0.51 (±0.08)

9.6 (±0.6)

77

CPG2(Q)3-H6

ND

No competition

ND

[125I]-VEGF

0.51 (±0.06)

ND

ND

  1. VEGFR2(ΔKD)m was captured on the 9E10 antibody immobilised in 96-well plates. The fusion proteins were incubated with the captured VEGFR2(ΔKD)m and binding was determined. We also used a competition RIA where binding of [125I]VEGF to VEGFR2(ΔKD)m occurs in the presence of competing fusion proteins. The rate of binding was also determined, by exposing the fusion proteins to immobilised VEGFR2(ΔKD)m for short times before washing out the protein and measuring how much fusion protein was bound.
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