Figure 3 | British Journal of Cancer

Figure 3

From: Cell adhesion to the extracellular matrix protein fibronectin modulates radiation-dependent G2 phase arrest involving integrin-linked kinase (ILK) and glycogen synthase kinase-3β (GSK-3β) in vitro

Figure 3

(A–F) An 18-h inhibition of PI3-K by LY294002 using concentrations from 0.1 to 100 μ M in combination with serum starvation resulted in a nonlinear and polystyrene (P)- or FN-independent dose–response relation for A549 (A) and CCD32 cells (D). Determination of apoptosis of A549 (B) or CCD32 cells (E) induced by LY294002 (LY) plus serum starvation (SD) on polystyrene or FN in comparison with untreated controls (nt) was performed by annexin-V staining and flow cytometric analysis. Cell viability was >90% in all the cases. The radiation-dependent clonogenic cell survival of A549 (C) and CCD32 cells (F) grown on FN or polystyrene was tested after an 18-h incubation with the PI3-K inhibitor LY294002 (50 μ M) in serum-free medium. The data indicate no significant change of the clonogenic survival of irradiated cells on FN, whereas on polystyrene the survival was further reduced by LY294002 at 2 and 6 Gy in A549 cells and at 6 Gy in normal lung fibroblasts significantly (P<0.05).

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