Figure 7 | British Journal of Cancer

Figure 7

From: Cell adhesion to the extracellular matrix protein fibronectin modulates radiation-dependent G2 phase arrest involving integrin-linked kinase (ILK) and glycogen synthase kinase-3β (GSK-3β) in vitro

Figure 7

Cyclin D1 expression and pRb phosphorylation were investigated after a 12-h LY294002 (50 μ M) incubation in A549 and CCD32 cells grown on polystyrene or FN in comparison with untreated controls (mock). Correlating the decreases of cyclin D1 expression and pRb phosphorylation with the DNA analysis shown in Figure 6 supported the important role of PI3-K-dependent growth factor pathways for the regulation of cell division and associated cell cycle proteins at FN presence and absence. Most interestingly, cells attached to FN did not demonstrate a downregulation of cyclin D1 expression or pRb phosphorylation, which indicates a strong influence of ECM on the cell cycle regulation via ILK-GSK-3β.

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