Figure 6

Effect of synthetic NESP55 peptide on Western blot analysis of NESP55 in EPT (benign insulinoma) and adrenal pheochromocytoma (PC, benign tumour). Neuroendocrine secretory protein 55 antiserum was adsorbed with a synthetic fragment of human NESP55 (GPIPIRRH) at 1 μ M overnight prior to incubation. In unadsorbed blots (−), the NESP55 immunoreactive protein migrated as a major band of 45–55 kDa and a minor band at 40 kDa. In adsorbed blots (+), all NESP55 immunolabelling was abolished.