Figure 5

Morphological changes and reorganisation of actin cytoskeleton on MDA-MB-231 cells treated by zoledronic acid (ZOL). – Comparison with C3 exoenzyme. The actin cytoskeleton of MDA-MB-231 cells was labelled by phalloidin-TRITC and analysed by confocal microscopy. On untreated cells (A), note the actin stress fibres and cell spreading. Treatment for 18 h with 1 μ M ZOL (B) induced morphological changes of cells associated with disorganisation of actin stress fibres. The modifications induced by ZOL were reversed by geranylgeraniol (GGOH) (C) but not by farnesol (FOH) (D) and mimicked by C3 exoenzyme (E) (Scale bar=10 μ M).