Figure 3
(A) Rt–PCR and Western blot analysis of γ-tubulin and STOP in MN1 (wt-p53) and MDD2 cells (mut-p53); (B) Western blot analysis of proteins interacting with microtubules; (C) Western blot analysis of STOP protein in HCT116 p53+/+ and p53−/− cells and negative regulation of the STOP promoter by wt-p53 in HCT1116 −/− colorectal cells. (A) and (B) represent one of the three experiments performed. Surv: survivin; Actin: β-actin. For (C), HCT116 p53-null colorectal cancer cells were cotransfected with the pGL-3-STOP-luciferase plasmid and pCMV-wt-p53 plasmid. Basic, pGL-3 basic plasmid; control, pGL-3 control plasmid; STOP+e, cotransfection of pGL-3-STOP-luciferase plasmid and empty pCMV; STOP+p53, cotransfection of pGL-3-STOP-luciferase plasmid and pCMV-wt-p53.
