Table 1 Parameters of dynamic instability in MN1 and MDD2 cells

From: Drug resistance associated with loss of p53 involves extensive alterations in microtubule composition and dynamics

Parameters

MN1 (wt-p53)

MDD2 (mut-p53)

Percentage of change a

Mean rate (μm min−1)

   

 Growth

8.6±0.9b

8.9±0.8

+1

 Shortening

29.1±2.8

21.7±1.7*

−25

Mean duration (min)

   

 Growth

0.29±0.03

0.32±0.04

+6

 Shortening

0.18±0.02

0.16±0.01

0

 Attenuation

0.51±0.04

0.35±0.03*

−29

Mean length change/event (μm)

   

 Growth

2.5

2.8

+12

 Shortening

5.2

3.5

−33

Percentage time spent

   

 Growth

25.4

39.7

+56

 Shortening

12.8

17.1

+34

 Attenuation

61.8

43.2

−30

 Dynamicity (μm min−1)

5.9

7.9

+33

  1. *P<0.02; estimates of significance by Student's t-test.
  2. aThe percentage difference relative to the corresponding parameter between MDD2 cells and MN1 cells. Parameters of dynamic stability were determined by measurement of at least 31 microtubules from seven to 12 cells for each cell type.
  3. bs.e.m.
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