Table 1 Amount of BRCA1 and BRCA2 proteins expressed by MCF-7, MDA-MB 231 and HBL 100 cells after treatment with 30 μ M resveratrol and 48 h exposure.

From: Resveratrol increases BRCA1 and BRCA2 mRNA expression in breast tumour cell lines

  

Proteins assayed (%)

 

Cell lines

BRCA1

BRCA2

MCF-7

Untreated controls

0.58±0.01

1.00±0.03

 

30 μ M resveratrol

0.51±0.02*

0.80±0.16*

MDA-MB231

Untreated controls

1.85±0.34

0.48±0.12

 

30 μ M resveratrol

1.80±0.32

0.41±0.02

HBL100

Untreated controls

0.95±0.04

0.90±0.09

 

30 μ M resveratrol

1.15±0.18

1.00±0.03

  1. BRCA1 and BRCA2 proteins were obtained by DNA-binding protein purification, specific immunoprecipitation with anti-BRCA1 (556445) or anti-BRCA2 (C-19) antibodies and protein A affinity chromatography and expressed in percentage calculated as follows: 100 × d.p.m. DNA-binding proteins bound specifically to antibodies raised against BRCA1 or BRCA2/d.p.m. of total DNA-binding proteins purified on heparin affinity chromatography. All data are expressed as mean±s.e. of three assays (*), P<0.05, vs untreated controls (Student's t-test).
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