Figure 1

Zoledronic acid inhibits the growth of PC cells. (A) The cell lines BxPC-3, CFPAC-1 and PANC-1 (3 × 10⁴ ml⁻¹) were seeded in 96-well plates and incubated for 24 h. After 24 h, medium was removed and replaced with medium containing increasing concentrations of ZOL (1–100 μ M). After 72 h of incubation, cell proliferation was determined by the MTT assay. A value of 100% was assigned to untreated control cultures, and the antiproliferative effects of ZOL were calculated as percentage of residual growth. Each point is the mean value of at least four replicate experiments ±s.e. (B) The IC₅₀ drug concentration for each cell line was measured by a logarithmic interpolate curve on the percentage of residual growth at each concentration point (C and D) Morphological changes induced in cell cultures after 72 h exposure to ZOL. Analysis was performed by inverted phase-contrast microscopy. (C) Untreated cultures; (D) treated cultures with ZOL (50 μ M).