Figure 2

Expression of β-catenin in TCC lines and normal uroepithelial cells. Expression of β-catenin (CTNNB1) was determined in the indicated TCC lines, NUEC and HepG2 hepatoma cells at the mRNA level (A) by RT–PCR using GAPDH for comparison and at the protein level (B) using α-tubulin for comparison. Control in (A) refers to PCR without cDNA, the size marker is shown on the right-hand lane. Control in (B) was supplied by the antibody manufacturer.