Table 1 Properties of TCC lines

TCC line	*TP53* mutation	RB Western blot	*CDKN2A*	*APC* methylation	*APC* *Western blot*	*CDH1* methylation	E-Cadherin Western blot
VmCub1	Mutant	+	Mutant	−	+	−	+
	Exon 5
VmCub2	Mutant	+	Deleted	−	+	−	−
	Exon 5
SW1710	Mutant	+	Deleted	−	+	±	−
	Exon 8
SD	Mutant	+	Deleted	−	+	−	+
	Exon 4
HT1376	Mutant	−	+	+	+	−	+
	Exon 7
5637	Mutant	−	+	−	+	−	±
	Exon 8
BFTC905	Wild-type	+	Deleted	−	+	−	+
	(MDM2↑↑)

For each cell line, TP53, RB and CDKN2A status are given as previously described (Steinhoff et al, 2002; Swiatkowski et al, 2002). APC and CDH1 hypermethylation and expression were determined in the present study as described in the Materials and methods section. Methylation status: + hypermethylated, − no hypermethylation; ± both hypermethylated and unmethylated alleles detectable; protein expression: + present; − not detectable; ± weakly detectable.

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