Figure 1

Establishment and characterisation of MCF7 human breast cancer cells expressing constitutively active RasG12V. (A) Parental MCF7 cells, control-vector-transfected MCF7 cells, and four selected MCF7 cell clones expressing RasG12V were cultured overnight in a medium containing 0.5% charcoal-stripped fetal bovine serum (CS-FBS). The cells were then harvested for Western blot analyses with various indicated antibodies. (B–E) The cells described in (A) were cultured for 5 days in a medium containing 10 or 0.5% fetal bovine serum (FBS) (B, C) or 10 or 0.5% CS FBS. Cells were counted with a Coulter counter.