Figure 2
From: Roles of the PI-3K and MEK pathways in Ras-mediated chemoresistance in breast cancer cells
Increased resistance of MCF7RasG12V breast cancer cells to treatment with doxorubicin, paclitaxel, and 5-fluorouracil. (A) The cells described in Figure 1 were pulse-exposed to doxorubicin, paclitaxel, or 5-fluorouracil for 4 h at the indicated concentrations. The cells were then cultured for an additional 7 days with a drug-free medium supplemented with 10% CS-FBS medium. Cell viabilities after chemotherapy were determined by an MTT assay as described in Materials and Methods and were expressed as the percentage of the value of untreated cells. (B) One representative cell clone (clone C) was treated with doxorubicin, paclitaxel, or 5-fluorouracil for 8 h, followed by additional culture for 16 h with 10% CS-FBS medium. Cells were harvested and subjected to apoptosis ELISA.
