Figure 3
From: Roles of the PI-3K and MEK pathways in Ras-mediated chemoresistance in breast cancer cells
Noncrossing inhibition of RasG12V-induced activation of PI-3K and MEK by their respective specific inhibitors (PD98059 and LY294002). MCF7RasG12V clone C cells were exposed to increasing concentrations of PD98059 as indicated (A) or increasing concentrations of LY294002 as indicated (B) in a medium containing 0.5% CS FBS for 16 h, accompanied by parallel cultures of parental MCF7 cells and control-vector- transfected cells in 0.5% CS FBS medium without PD98059 or LY294002. Cells were collected for Western blot analysis for the levels of Ras (a), phosphorylated Akt (b), total Akt (c), phosphorylated MAPK (d), and MAPK (p42) (e).
