Figure 2

(A) Erk-MAP kinase activity in HCT116 cell lines. Cell lysates used for in vitro kinase assay were analysed by equal protein loading on 10% SDS–PAGE (under nonreducing conditions) followed by Western blotting using antibodies against phosphorylated Erk-MAP kinase (E10 antibody, New England Biolabs) and (B) total Erk (Santa Cruz, CA, USA).