Figure 3 | British Journal of Cancer

Figure 3

From: Downregulation of urokinase plasminogen activator receptor expression inhibits Erk signalling with concomitant suppression of invasiveness due to loss of uPAR–β1 integrin complex in colon cancer cells

Figure 3

(A) Expression of HMW-uPA in conditioned medium of WT, mock- and A/S-transfected HCT116 cell lines. Conditioned medium was analysed by equal protein loading on 10% SDS–PAGE (under nonreducing conditions) followed by Western blotting using monoclonal anti-uPA antibody. Ukidan was used as standard reference uPA (Serono, Australia). The expression of HMW-uPA was quantified by densitometry and is expressed as peak optical density (peak OD). Results are representative of three experiments. (B) Gelatin zymography showing the amounts of pro-MMP-2 and pro-MMP-9 secreted in conditioned medium (concentrated 90–100-fold) from HCT116 cell lines. The positions of purified pro-MMP-2 and pro-MMP-9 are shown on the left. Results are representative of three independent experiments.

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