Figure 4

Effect of uPA on the activation of Erk. Subconfluent cultures of mock and A/S HCT116 cells were serum starved for 24 h, acid stripped for 1 min and incubated with uPA (20 nM) for 30 min. The level of phospho Erk1/2 was determined by Western blot using equal protein loading. Results are representative of one experiment. The experiment was repeated three times.