Table 4b (b): Effect of uPA, MMPs and plasmin inhibitors on the matrix degradation of mock-transfected HCT116 cell line

From: Downregulation of urokinase plasminogen activator receptor expression inhibits Erk signalling with concomitant suppression of invasiveness due to loss of uPAR–β1 integrin complex in colon cancer cells

Cell treatment

Matrix degradation (CPM/3 × 10 5 cells)

−Plg

275±32

+Plg

600±56

+Plg+DMSO

586±37

+Plg+amiloride (2 mM)

263±42*

+Plg+phenanthroline (2 mM)

260±33*

+Plg+Trasylol (10 μg ml−1)

270±30*

+Plg+IgG2a (10 μg ml−1)

586±60

+Plg+anti-uPAR (10 μg ml−1)

426±40*

Matrix degradation of the cells was measured by the release of [3H]-collagen IV in the medium. Results are shown as mean±S.E.M of three different experiments performed in triplicate. *Significantly different, P<0.001, compared to control cells in the presence of Plg.

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