Figure 1
From: Involvement of nucleophosmin/B23 in TPA-induced megakaryocytic differentiation of K562 cells
Effect of TPA on cell growth and differentiation of K562 cells. (A) The growth curve after 20 nM TPA treatment for various times (24–72 h). Cultures were harvested and cell numbers were counted with a haemocytometer. Points, means of triplicate±SD. Viability of the cells was >90% under these TPA treatments. (B,C) K562 cells were treated with 20 nM TPA for 72 h and the differentiation was assayed by measuring the expressions of differentiation markers. Expressions of the CD41a or CD42b were evaluated by flow cytometry using specific anti-CD41a or anti-CD42b FITC-conjugated monoclonal antibody.
