Figure 5
From: Involvement of nucleophosmin/B23 in TPA-induced megakaryocytic differentiation of K562 cells
Assessment of cellular response of nucleophosmin/B23 wild-type and deletion mutant overexpressed cells to TPA-induced differentiation. The parental K562 cells, the control vector-transfected K562 cells (K562/vector) and the stable clones (two for each) of nucleophosmin/B23 wild-type (K562/B23) or deletion mutants (K562/D1, K562/D2 or K562/D3) were treated with 20 nM TPA for 72 h. After the cells were harvested, expressions of CD41a (A) or CD42b (B) were evaluated by flow cytometry using specific anti-CD41a or anti-CD42b FITC-conjugated monoclonal antibody. Bars, means of triplicate±SD. *P<0.05, as compared with K562/vector cells under same TPA treatment.
