Figure 2
From: Functional analysis of the actin-binding protein, tropomyosin 1, in neuroblastoma
Exogenous TM1 expression does not alter the actin cytoskeleton. Vector (A,C) and TM1 (B,D) transfected IMR32 (A,B) and BE(2)-C (C,D) cells were grown on coverslips. Cells were then stained with TritC-phalloidin to detect actin microfilaments. Panels (B,D) show the same cells stained with α-f/9d to detect cells expressing TM1. The percentage of TM1-transfected cells calculated to be TM1-positive with α-f/9d staining was 36% IMR32 cells and 67% BE(2)-C cells.
