Figure 3
From: Functional analysis of the actin-binding protein, tropomyosin 1, in neuroblastoma
Exogenous TM1 localises to both the detergent-insoluble- and -soluble-fractions. Equal volumes of detergent-soluble and -insoluble fractions of control (C), vector-transfected (V) and TM1-transfected (TM1) cells were loaded into each lane of an SDS–polyacrylamide gel. Western blots were probed with α-f/9d to detect TM1 as indicated. (A) Lysates from IMR32 cells and (B) Lysates from BE(2)-C cells. *Note that after a longer exposure, TM1 can be detected in the insoluble fraction of the IMR-32 TM1-transfectants. (C) Fractionated extracts from SH-EP cells showing the detergent-soluble fraction (S), the insoluble fraction (I) and the whole-cell lysate (W). Western blots of lysates were probed with α-f/9d and actin, as indicated.
