Figure 2
From: TNF-α increases human melanoma cell invasion and migration in vitro: the role of proteolytic enzymes
Representative gelatin zymograph of control and TNF-α-stimulated HBL melanoma cell extracts and cell conditioned medium. Cells constitutively expressed pro-MMP-2 (66.3 kDa band) in the culture medium and no upregulation or activation of the latent enzyme was observed after preincubation with TNF-α at 200 U ml−1. Lanes 1 and 2 represent internal human purified MMP-2 and MMP-9 standards.
