Table 1 Effect of TNF-α on the general protease activity of HBL cells

From: TNF-α increases human melanoma cell invasion and migration in vitro: the role of proteolytic enzymes

 

Net fluorescence units

TNF- α concentration (U ml−1)

Cell extracts

Cell conditioned medium

0

5699±228

3776±118

100

6057±146

2726±759

250

4857±270

2908±771

500

6066±288

3929±532

1000

5114±744

2458±437

  1. Cells cultured in a 24-well plate were stimulated with the relevant TNF-α concentration for 24 h prior to cell extraction and collection of cell conditioned medium for measurement of protease activity using the quenched fluorescent substrate assay. Results are the mean±s.e.m. of two separate experiments.
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