Table 1 Primary antibodies used for immunocytochemistry and Western blotting
Antibody | Dilution, immuno-fluorescence | Dilution, immuno-peroxidase | Dilution, Western blotting | Clone | Cat. no | Company |
|---|---|---|---|---|---|---|
VMAT1 (goat) | 1 : 100 | 1 : 1000 | 1 : 2000 | sc-7718 | Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA | |
VMAT1 (rabbit) | 1 : 100 | 1 : 10 000 | — | Gift from Jeffrey D Erickson, NIH, MD, USA | ||
VMAT2 (rabbit) | 1 : 100 | 1 : 10 000 | — | Jeffrey D Ericksson | ||
VMAT2 (rabbit) | 1 : 100 | 1 : 1000 | 1 : 2000 | AB1767 | Chemicon, Temecula, CA, USA | |
CgA (mouse) | 1 : 500 | 1 : 1000 | — | LK2H10 | 1199021 | Boehringer Mannheim, Mannheim, Germany |
SV2 (mouse) | 1 : 20 | — | — | Development Studies, Hybridoma Bank |
